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KMID : 1138520030060020149
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Journal of Pharmacopuncture
2003 Volume.6 No. 2 p.149 ~ p.158
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The Effects of Bee Venom on PLA_2 and Calcium Concentration in Raw 264.7 Cells
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Yun Jong-Il
Jo Hyun-Chol
Sim Sung-Yong
Kim Kee-Hyun
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Abstract
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Objective : The purpose of this study was to investigate the effect of Bee Venom on the lipopolysaccharide, sodium nitroprusside and hydrogen peroxide induced expression phospholipase A_2 and calcium concentration in RAW 264.7 cells, a murine macrophage cell line.
Method : The expression of phospholipase A_2 was determined by western blotting with corresponding antibodies, and the generation of intracellular calcium concentration was investigated by delta scan system in RAW 264.7 cells.
Results : 1. Compared with control, expressions of lipopolysaccharide-induced phospholipase A_2 were decreased significantly by 1;{mu}g/{mu}l of bee venom and decreased by 0.5, 5;{mu}g/{mu}l of bee venom. 2. Compared with control, expressions of sodium nitroprusside-induced phospholipase A_2 were decreased significantly by 5;{mu}g/{mu}l of bee venom but increased by 0.5, 5;{mu}g/{mu}l of bee venom. 3. Compared with control, expressions of hydrogen peroxide-induced phospholipase A_2 were decreased significaltly by 1{mu}g/{mu}l of bee venom and decreased by 0.5;{mu}g/{mu}l of bee venom but increased by 5;{mu}g/{mu}l of bee venom. 4. Compared with control, lipopolysaccharide, sodium nitroprusside and hydrogen peroxide- induced intracellular calcium concentrations were decreased by 0.5, 1, 5;{mu}g/{mu}l of bee venom and by indomethacin
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KEYWORD
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Bee Venom, PLA_2, Calcium, RAW 264.7 cell
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